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P07.01

POTENTIAL DIAGNOSTIC AND PROGNOSTIC VALUE OF
LYMPHOCYTIC MITOCHONDRIAL DNA DELETION IN
RELATION TO FOLIC ACID STATUS IN HEPATOCELLULAR
CARCINOMA

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Dalia Omran , Abdel-Rahman Zekri , Hosny Salama , Eman Medhat , Sherif
Hamdy , Yasser M. Bakr , Amira youssef , Doaa Saleh 3
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1 Department of Endemic Medicine and hepatology, Cairo University, NCI, Cairo University,
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Cairo, Egypt
Corresponding author’s email: daliaomran@kasralainy.edu.eg
Introduction: Hepatocellular carcinoma (HCC) is one of the most leading causes of
death worldwide.
Aims: studying mtDNA deletion as diagnostic and prognostic molecular marker in
relation to serum folic acid level in Egyptian patients with HCV related HCC. ePOSTER ABSTRACTS

Material and Methods: The prospective case control study was conducted on ninety
adult patients; 50 patients with HCC, 20 with liver cirrhosis (LC) and 20 with chronic
hepatitis C (CHC), in addition to 10 healthy subjects. Serum folic acid was measured
using ELISA and lymphocytic mtDNA deletions was measured using real-time PCR.
HCC patients were followed up over a period of one year dating from initial presentation.
The diagnostic accuracy of mtDNA deletions frequency was evaluated using receiver-
operating characteristic (ROC) curve analysis. Correlations between different variables
were calculated using Spearman’s Correlation Coefficient. Survival analysis was analyzed
with the Kaplan-Meier method.

Results: There was a significant elevation of mtDNA deletions and a significant decrease
in serum folic acid in HCC group compared to other groups (P: <0.01& P< 0.05
respectively).
Lymphocytic mtDNA deletions had a sensitivity of 82%, specificity of 60% at cut off
(ΔCt) 2.65 with AUC 0.818 and 95% CI; (0.72-0.9) for HCC diagnosis.
Regarding the clinicopathological features of HCC, we found that mtDNA deletions
frequency significantly correlated with HCC size (r= 0.9,P <0.01) but none significantly
with the number of HCC nodules (r=0.0240, P:0.8) and serum AFP level (r=0.16,
P:0.26). Serum folate level negatively correlated with HCC size, foci number, serum AFP

EASL HCC Summit • Geneva, Switzerland • 2-5 February, 2017 243