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P07.06

ASS1 IMMUNOHISTOCHEMISTRY IDENTIFIES
UNCLASSIFIED HEPATOCELLULAR ADENOMA.
EXPERIENCE OF A SINGLE FRENCH LIVER CENTER

4
1, 2
Anne-Aurélie Raymond , Jean-Frédéric Blanc , Jean-William Dupuy , Brigitte
1, 3
Le Bail , Nathalie Dugot-Senant , Charles Balabaud , Paulette Bioulac-Sage * 1,
1
1, 5
6
5 , Frédéric Saltel 1, 2
3
1 Bariton Inserm 1053, Bordeaux, France, Oncoprot, INSERM 1053, Bordeaux, France, Service
2
Hépatologie Gastroentérologie et Oncologie Digestive, Hôpital Haut Lévêque, CHU Bordeaux,
4 Plateforme Protéome, Centre de Génomique Fonctionnelle de Bordeaux, Université de Bordeaux,
Bordeaux, France, Service de pathologie, Hôpital Pellegrin, CHU Bordeaux, Bordeaux, France,
5
6 Plateforme d’histopathologie, INSERM 005, Bordeaux, France, Bordeaux, France
Corresponding author’s email: charles.balabaud@u-bordeaux.fr
Introduction: In our center all hepatocellular adenoma (HCA) are classified routinely
by immunohistochemistry. Unclassified HCA (UHCA) are defined by default (HCA
markers negative). ePOSTER ABSTRACTS

Aims: To test UHCA with ASS1 antibodies (a protein belonging to the Arginine
metabolic pathway), a marker identified by proteomic analysis in UHCA and to correlate
with clinicopathological data.
Material and Methods: From a total of 218 resected cases (189F/29M) there were 70
H-HCA, 69 IHCA, 22 b-HCA [15 ex3], 32 b-IHCA [19 ex3], 15 UHCA and 10 not
classifiable (massive hemorrhage/necrosis in 8 cases, waiting identification in 2 cases).
ASS1 IHC was performed in all UHCA, 3 HCA of each other subtype and 3 focal nodular
hyperplasia (FNH).

Results: All 15 UHCA were women, mean age 40 (range 27-48); all on oral contraceptives,
mean of 22 years (range 11-30) in 10/15 cases. BMI was raised in 11 cases, mean 30
(range 19.7- 45.7). Mode of discovery was death by hemorrhagic rupture of the liver,
severe hemorrhage (intratumoral, intrahepatic, or peritoneal), pain, and chance in 1, 5,
6, and 3 cases respectively.The number of nodules was 1, 2, or ≥ 5 in 9, 4 and 2 cases
respectively. Associated diseases were diabetes type 2, arterial hypertension in 4 and 3
cases (one of renal origin) respectively. Non tumoral liver (NT) was steatotic in 10 cases:
≥ 60%, 30-60%, 10-30% in 6, 2 and 2 cases respectively. Areas of hemorrhage, small or

EASL HCC Summit • Geneva, Switzerland • 2-5 February, 2017 251