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P07.07-YI

PERIPHERAL T CELL SUBPOPULATION IN PATIENTS
WITH HEPATOCELLULAR CARCINOMA: RELATION TO
ABLATION THERAPY

1
1
* 1
Walid ElSherbiny , Raghda E Farag , Shaker W. Shaltout , Nashwa K. Abousamra 2
1 Tropical Medicine Department, Mansoura University Hospital, Clinical Pathology Department,
2
Faculty of Medicine, Mansoura, Egypt
Corresponding author’s email: shakershaltout2@yahoo.com

Introduction: Locoregional therapy has become increasingly important for patients
with HCC because of advances in techniques, survival benefit, and a favorable safety
profile. Ablative techniques share, irrespective of their mechanism to induce cell death, the
ability to stimulate immunological responses. These responses are validated through the
measurement of peripheral immune cells in the systemic circulation.
Aims: To investigate changes in the peripheral immune cells presented in CD4, CD8 and
CD4/Cd8 ratio after HCC ablation by different procedures and the relation between these ePOSTER ABSTRACTS
changes and ablation result.

Material and Methods: This study investigated 73 HCC patients who were admitted
to Tropical Medicine Department at Mansoura University Hospital, Egypt. The patients
were stratified into three groups according to ablative technique used. RFA was performed
for24 cases, MWA for24 and TACE for 25 cases. All patients underwent full history
taking, clinical examination, full basic investigations, triphasic abdominal computerized
tomo-graphy before and 4 weeks after the treatment and lymphocyte subset assay by flow
cytometry 1 day before, and 4 weeks after the treatment. Then each group subdivided into
successful and unsuccessful subgroup according to the result of ablation by CT.

Results: After treatment, CD4+ cells and CD4/CD8 ratio remarkably increased (P
<0.001), and the CD8+ cells significantly decreased (P <0.002) with concomitant increase
in the CD4+/CD8+ ratio (P < 0.001) in the RFA group; CD4+ cells markedly increased
after treatment (P <0.001), with increase in CD4/CD8 ratio (P<0.007) but there were no
significant differences in CD8+ cells in the MWA group; the CD4+ cells and CD4/CD8
ratio dramatically decreased after treatment (P <0.001), and the CD8+ cells increased
significantly (P <0.001)in the TACE group. After division of each group to successful

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